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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
Grown as monolayer
fibroblast-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: lung, embryonic
Susceptibility to viruses: cytomegalovirus * poliovirus 1 * vesic. stomatitis (Indiana)
PROPERTIES: biochemistry; cell biology; genetics; enzymology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 137, IZSBS: BS CL49, ECACC: 87042207
Bibliographic references:
- Arch Virol 1982;72:127 - PMID: 6285862
- Proc Soc Exp Biol Med 1968;128:772 - PMID: 5668122
SPECIES: human, Black
TISSUE/ORGAN: lung, embryonic
ECACC catalog code: 87042207
Morphology: Fibroblast
Susceptibility to viruses: cytomegalovirus * poliovirus 1 * vesic. stomatitis (Indiana)
Description: Human Negroid embryo lung
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Enzymatic studies
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Limited lifespan.
Passages: 19;
Karyotype: 2n = 46, diploid
Derived from embryonic lung tissue of a Negro male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 137, IZSBS: BS CL49
Bibliographic references:
- Arch Virol 1982;72:127 - PMID: 6285862
- Proc Soc Exp Biol Med 1968;128:772 - PMID: 5668122
IZSBS catalog code: BS CL49
Grown as monolayer
fibroblast-like morphology
SPECIES: human, Caucasian (male)
TISSUE/ORGAN: lung, embryonic
Susceptibility to viruses: cytomegalovirus * poliovirus 1 * vesic. stomatitis (Indiana)
PROPERTIES: biochemistry; cell biology; genetics; enzymology
Available in the following LABORATORY:
Availability in cell line catalogues: ATCC: CCL 137, ECACC: 87042207
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295
Email: assunta.manniello@istge.it