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ICLC catalog code: HTL96026
Continuous culture, grown as monolayer
large spindle-shaped cells morphology
SPECIES: human (female, 67 years old)
TUMOR: carcinoma, undifferentiated
DEPOSITOR: Obtained from ECACC.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:4 once a week using trypsin/EDTA. Doubling time is about 43 hours. Seed at 2-4 x 104 cells/cm2; cell harvest is about 2 x 106 cells/150 cm2 flask. 37C, 5% CO2. , mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as human with AST, MD)
Availability in cell line catalogues: ECACC: 94090183, DSMZ: ACC 133
- Biochem Biophys Res Commun 1994;198:948-954 - PMID: 8117301
- Cancer Res 1992;52:1369 - PMID: 1737400
- Int J Oncol 1994;4:583-586
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-295