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SPECIES: human, Caucasian
TISSUE/ORGAN: skin, fibroblast
PATHOLOGY: cri du chat syndrome#123450 (OMIM record)
ECACC catalog code: 90102533
Description: Human Caucasian skin deletion in chromosome 5
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Chromosome studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
McCoy's 5a + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37C. Cells should not be centrifuged.
Karyotype: 2n = 46, diploid
Derived from the skin of an adult female Caucasian with Cri du Chat syndrome. The cells have a deletion in chromosome 5 and have a finite lifespan; up to 35 population doublings expected.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 90
- Cytogenetics 1966;5:137 - PMID: 5921496
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293