3T6

Version 4.0.200811

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3T6 (mouse, Swiss albino, fibroblast)

SPECIES: mouse, Swiss albino
TISSUE/ORGAN: fibroblast

DSMZ catalog code: ACC 202
Morphology: fibroblasts growing adherently as monolayer
Susceptibility to viruses: herpes simplex * pseudorabies * vaccinia * vesic. stomatitis (Indiana)
Description: Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse embryos in 1963; cells were described to secrete collagen and hyaluronic acid (= ATCC CCL 96) confirmed as mouse with IEF of AST, G6PD, MDH, NP
Viruses: ELISA: reverse transcriptase positive
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
85-90% Dulbecco's MEM + 10-15% FBS split confluent culture every 2-3 days using trypsin/EDTA, seed out about 2 x 106 cells/175 cm 2 at 37C with 5-10% CO2 cell harvest of about 15 x 106 cells/175 cm 2; doubling time of ca. 15 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (murine flat-moded hypertriploid-hypotetraploid karyotype; 67(64-73)<3n>) * isoenzymes (confirmed as mouse with IEF of AST, G6PD, MDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CCL 96, IZSBS: BS CL 83

Bibliographic references:

J Cell Biol 1963;17:299-313
Virology 1965;27:179-185 - PMID: 4284655