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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL96005
Continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: monkey, African green
TISSUE/ORGAN: kidney
Original line: CV-1
TRANSFORMED BY: SV40
DEPOSITOR: Dr T. Florio, Neuroscience Dept, ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: The line contains T antigen. Suitable for transfection by vectors requiring expression of SV40 T antigen.
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:3-1:6 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as monkey with MD, NP)
Availability in cell line catalogues: IZSBS: BS CL103, ECACC: 87021302, DSMZ: ACC 60
Bibliographic references:
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it