COS-7

Version 4.0.200811

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COS-7 (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

DSMZ catalog code: ACC 60
Morphology: fibroblast-like cells growing as monolayers
TRANSFORMED BY: SV40
Description: African green monkey kidney derived from CV-1, a simian cell line (cercopithecus aethiops), by transformation with an origin-defective mutant of SV-40; cells were described to support the growth of SV-40 viruses; classified as risk category 1 according to the German Central Commission for Biological Safety (ZKBS) confirmed as of primate origin with IEF of LDH, MPI, NP, PEP B
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Dr. R. Riebe, Friedrich-Loeffler-Institute, Insel Riems, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% Dulbecco's MEM + 10% FBS split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 1 x 106 cells/80 cm 2 ; cells are growing slowly at 37C with 5% CO2 about 2-3 x 106 cells/80 cm 2; doubling time of ca. 35-48 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (simian hypodiploid karyotype with 25% polyploidy; 57(53-58)<2n>) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile (identical to COS-1)) * isoenzymes (confirmed as of primate origin with IEF of LDH,MPI,NP,PEP B)

Availability in cell line catalogues: ATCC: CRL 1651, IZSBS: BS CL103, ECACC: 87021302, ICLC: ATL96005

Bibliographic references:

Cell 1981;23:175-182 - PMID: 6260373