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SPECIES: monkey, African green
ECACC catalog code: 87021302
TRANSFORMED BY: SV40
Description: Monkey African green kidney, SV40 transformed
Viruses: See Properties
Depositor: Dr H R B Pelham, MRC Laboratory of Molecular Biology, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus replication studies: SV40
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Derived from CV-1simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1651, IZSBS: BS CL103, DSMZ: ACC 60, ICLC: ATL96005
- Cell 1981;23:175-182 - PMID: 6260373
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293