COS-7

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

COS-7 (monkey, African green, kidney)

SPECIES: monkey, African green
TISSUE/ORGAN: kidney

ECACC catalog code: 87021302
Morphology: Fibroblast
TRANSFORMED BY: SV40
Description: Monkey African green kidney, SV40 transformed
Viruses: See Properties
Depositor: Dr H R B Pelham, MRC Laboratory of Molecular Biology, Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus replication studies: SV40

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 5; Derived from CV-1simian cells transformed by an origin-defective mutant of SV40. The cells support the growth of recombinant SV40 viruses. The cells should be handled at laboratory containment level 2.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1651, IZSBS: BS CL103, DSMZ: ACC 60, ICLC: ATL96005

Bibliographic references:

Cell 1981;23:175-182 - PMID: 6260373