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TISSUE/ORGAN: lymphocyte, B
TUMOR: adenocarcinoma, ovary
ECACC catalog code: 93052623
Description: Human normal B lymphocytes
Viruses: See Properties
Depositor: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C.
Normal B lymphoid cell line derived from the peripheral blood of a 74 year old Chinese female (the same patient as COLO 720E) with ovarian cancer. These B-lymphoid cells grow as characteristic clumps of cells in the media. Often the peripheral cells of the clumps have their spike-like uropods extending into the media.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293