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SPECIES: fish - carp, cyprinus
ECACC catalog code: 95070628
Description: Carp leukocyte
Viruses: See Properties
Depositor: Prof W Ahne, Institute of Zoology, Fishery Biology and Fish Diseases, University of Munich, GERMANY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Immunological research, fish virology, fish cell physiology.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) or TC-199 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Spilt confluent cultures 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 25-28C.
Karyotype: 2n = 104
The cell line CLC (Carp Leukocyte Culture) was established from peripheral blood mononuclear cells of a normal 2-year-old female common carp, Cyprinus carpio. They possess phagocytic activity and are reported to be stimulated by TNF, gamma-interferon and LPS therefore resembling characteristic features of monocytes / macrophages. CLC replicate fish viruses e.g. IPNV, PFR, SVCV and VHSV.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Dev Comp Immunol 1990;14:255
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293