CHO-K1

Version 4.0.200811

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CHO-K1 (hamster, Chinese, ovary)

SPECIES: hamster, Chinese
TISSUE/ORGAN: ovary

ECACC catalog code: 85051005
Morphology: Epithelial
Susceptibility to viruses: arbovirus * Getah arbovirus * mengovirus * vesic. stomatitis (Indiana)
Description: Hamster Chinese ovary
Viruses: See Properties
Depositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Nutritional and gene expression studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 17; Karyotype: Hypodiploid, modal No 20
A subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline due to the absence of the gene for proline synthesis, the block in the biosynthetic chain lies in the step converting glutamic acid to glutamine gamma serialdehyde. They undergo morphological changes in response to cholera toxin.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 61, DSMZ: ACC 110, ICLC: ATL98003

Bibliographic references:

J Exp Med 1958;108:945
Proc Natl Acad Sci U S A 1965;52:288 - PMID: 5244736
Proc Natl Acad Sci U S A 1968;60:1275 - PMID: 5244736
Virology 1965;27:11 - PMID: 4283990