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SPECIES: hamster, Chinese
ECACC catalog code: 85050302
Description: Hamster Chinese ovary
Viruses: See Properties
Depositor: Dr R Downing, PHLS CAMR, Porton Down, Salisbury, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Nutritional and gene expression studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Ham's F12 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: Hypodiploid, modal No 20
A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ICLC: ATL95003
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293