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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL06001
Continuous culture, grown as monolayer
undifferentiated morphology
SPECIES: mouse, 129/SV (male)
TISSUE/ORGAN: testis
TUMOR: teratocarcinoma
DEPOSITOR: Dr S. Astigiano, Transgenic Animals Dept, IST at ABC, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: pluripotent; differentiation
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM (with 4.5 g/l glucose) + 10% Calf serum; Freezing medium: 95% FCS + 5% DMSO; split 1:5 - 1:10, 3 times per week; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Growth on gelatin-coated dishes. Differentiation into visceral or parietal endoderm with retinoic acid or HMBA treatment
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as mouse with cytochrome c oxidase subunit I (cox I) primers)
Bibliographic references:
- Cell 1975 ;5(3):229-43 - PMID: 1097123
- Differentiation 1984;27(3):236-42 - PMID: 6500205
- Exp Cell Res 1979;124(2):381-91 - PMID: 510422
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it