|
Version 4.0.200811 |
|
This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL03004
Continuous culture, grown as suspension
round cells, erythromegakaryocytic morphology
SPECIES: human (male, 14 year old)
TISSUE/ORGAN: bone marrow
TUMOR: leukemia
Derived from: a patient with acute myeloid leukemia with a complete erythro-megakaryocytic phenotype
DEPOSITOR: Prof. G.P. Bagnara, Institute of Histology and Embryology, University of Bologna, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: Expression of both c-mpl and erythropoietin receptor (Epo-R). Spontaneous B1647 cell proliferation is significantly potentiated by thrombopoietin (TPO)
Available in the following LABORATORY:
-
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
Iscove's modified Dulbecco's medium + 10% FBS + 2mM L-Glutamine ; Freezing medium: Culture medium + 70% FBS + 10% DMSO; Split 1:3-1:6; 37C, 5% CO2 Passage: >50, mycoplasma negative, HOECHST and PCR
Validated by: Multiplex PCR (tested against human, rat, mouse primers. Confirmed as human with cytochrome c oxidase subunit I (cox I) primers)
Bibliographic references:
- Br J Haematol 1997;98:549-559 - PMID: 9332307
- Haematologica 2002;87:1242-1247 - PMID: 12495897
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it