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ICLC catalog code: HTL01015
Continuous culture, grown as suspension
round, multiformed cells, some giant cells morphology
SPECIES: human (male, 64 year old)
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
DEPOSITOR: Dr A. De Lerma Barbaro, Clinical and Biological Sciences, University of Insubria, Varese, Italy.
Cell line available for distribution. For non-commercial investigative use only
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate + 1% non-essential amino acids + 9 ug/ml bovine insulin ; Freezing medium: 90% FBS + 10% DMSO; maintain cultures between 3x10^5 and 1x10^6 cells/ml; split 1:3-1:6; 37C, 5% CO2, mycoplasma contamination eliminated with ciprofloxacin; negative Hoechst and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Availability in cell line catalogues: DSMZ: ACC 124
- Int J Cancer 1988;41:456-461 - PMID: 3162233
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293