CF2Th

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

CF2Th (dog, thymus)

SPECIES: dog
TISSUE/ORGAN: thymus

ECACC catalog code: 90110521
Morphology: Epithelial
Susceptibility to viruses: calicivirus * feline rhinotracheitis * herpes simplex * parvovirus * pseudorabies * reovirus * retroviruses
Description: Canine thymus
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: C type viruses, xenotrophic viruses

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 0.02% EDTA: 5% CO2; 37C.
Passages: 62; Used widely for the growth of C type viruses obtained from a variety of species including cat, baboon and humans. Also permissive for xenotrophic viruses found in BALB/c and AKR mice.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1430, IZSBS: BS CL13, ICLC: AL97003

Bibliographic references:

Am J Vet Res 1983;44:865 - PMID: 6869994
Arch Virol 1983;76:257 - PMID: 6307228
Avian Dis 1984;28:216 - PMID: 6721794
In Vitro 1976;12:665 - PMID: 190163
In Vitro 1977;9:176