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SPECIES: fish - Ictalurus punctatus (catfish)
ECACC catalog code: 95060212
Description: Channel Catfish ovary
Viruses: See Properties
Depositor: Dr J Plumb, Department of Fisheries and Allied Aquacultures, Auburn University, Auburn, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Isolation of channel catfish virus
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 30C.
CCO was established from the ovaries of a healthy, juvenile channel catfish, Ictalurus punctatus. The cells grow at temperatures from 10C to 35C with the optimum at 30C and with a reported population doubling up to 12 hrs. CCO cells replicated channel catfish virus but were refractory to 12 other viruses from fish, birds and mammals.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Can J Fish Aqaut Sci 1980;37:871
- In vitro 1980;16:365 - PMID: 7390534
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293