CCF-STTG1

Version 4.0.200811

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CCF-STTG1 (human, Caucasian, brain, astrocytoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: brain
TUMOR: astrocytoma

ECACC catalog code: 90021502
Morphology: Astrocytoma
Description: Human Caucasian astrocytoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: HLA profiles

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established from Grade IV astrocytoma from 68-year old Caucasian female. Cells exhibit strong perinuclear acid and phosphatase activity, glial fibrillary acidic protein present in 70-80% cells. HLA-DR present on approx. 20-30% cells after 48 hours culture.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1718, IZSBS: BS TCL 125

Bibliographic references:

Fed Proc 1984;43:781
In Vitro 1983;19:275
J Neuroimmunol 1985;10:151 - PMID: 3877740