This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: mouse, AKR
ECACC catalog code: 94031010
TRANSFORMED BY: Radiant Agent Back To Radiant Agents' WebPage
Description: Mouse (AKR) thymus thymoma
Viruses: See Properties
Depositor: Dr D Iannelli, University of Naples, School of Agriculture, ITALY (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of the assembly and transport of Ly-2/3 antigen to the cell surface or to define the regions of Ly-2/3 interacting with the corresponding antibodies and with MHC class I molecules.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 10% Horse Serum (HS). Maintain cultures between 3-9x100,000 cells/ml; 5-10% CO2; 37C.
The parent cell line AKR/1a murine T cell line was exposed to a dose of 500 rads from an X-ray source. The mutant Lyt2-cells selected out by magnetic bead separation.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Immunogenetics 1994;40:151
- J Immunol Methods 1992;154:211 - PMID: 1401954
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293