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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: HTL01005
Continuous culture, grown as monolayer
epithelial-like morphology
SPECIES: human, Caucasian (female, 70 years old)
TISSUE/ORGAN: pleural effusion
TUMOR: mesothelioma
DEPOSITOR: Dr A.M. Orengo, Immunopharmacology Dept, IST, Genoa, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: IL-6 and TGF-beta2 production. Tag protein expression in the nucleus. CAM5.2+, HBME-1+
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:3 using trypsin/EDTA; 37C, 5% CO2 Passage: 54, mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR)
Bibliographic references:
- Eur Respir J 1999;13:527-534 - PMID: 10232421
- Int J Cancer 1998;78:740-749 - PMID: 9833768
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it