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SPECIES: human, Caucasian
TUMOR: leukemia, promyelocytic
ECACC catalog code: 96100920
Description: Human Caucasian promyelocytic leukaemia
Viruses: See Properties
Depositor: Dr D W Golde, Memorial Sloan-Kettering Cancer Centre, New York, USA (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of production and function of human eosinophils, analysis of differentiation.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Fetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use. Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37C.
Eos-HL-60 is a variant of the promyelocytic cell line HL60 (ECACC No. 85011431) established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.
Research council deposit: No;
Price Code: C;
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293