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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
L6H2 (rat)
ICLC catalog code: ATL00001
Continuous culture, grown as monolayer
fibroblast-like and round cells lightly adherent morphology
SPECIES: rat ( newborn)
non myogenic subclone
Original line: L6 (ATCC CRL1458)
DEPOSITOR: Dr S. Adamo, Medical Istology Embriology Dept, University La Sapienza, Rome, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: L6H2 display limited adhesion, do not give rise to myotubes, grow in soft agar
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split cultures 1:10-1:20 using Trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2 Passage: 7, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with MD, NP)
Bibliographic references:
- Am J Physiol 1993;265:C113-C121 - PMID: 8393277
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it