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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL99012
Continuous culture, grown as monolayer
oligodendrocyte morphology
SPECIES: rat, Sprague-Dawley (female, 2 week embryo)
TISSUE/ORGAN: brain
TRANSFORMED BY: murine leukemia virus
DEPOSITOR: Prof. T. Russo, Biochemistry and Medical Biotechnology Dept, Naples, Italy
Cell line available upon consent of the depositor. For non-commercial investigative use only
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:10-1:20 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as rat with NP, MD, MPI)
Bibliographic references:
- Neurosci Lett 1993;159:159-162 - PMID: 8152593
See also
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it