GI-ME-N

Version 4.0.200811

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GI-ME-N (human, Caucasian, bone marrow, metastatic neuroblastoma, stage IV)

ICLC catalog code: HTL98011
Continuous culture, grown as monolayer
neuroblast-like morphology
SPECIES: human, Caucasian (female, 2 years old) Karyology: chromosome marker: 1p del
TISSUE/ORGAN: bone marrow
TUMOR: metastatic neuroblastoma, stage IV
highly tumorigenic in nude mice
DEPOSITOR: Dr G.P. Tonini, Population Genetics Dept, IST at ABC, Genoa, Italy
Cell line available for distribution upon consent of the depositor. For non-commercial investigative use only
PROPERTIES: undetectable N-myc expression; the cells do not express HLA Class I

Available in the following LABORATORY:

Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
RPMI 1640 + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 2-4 x 10^4 cells/cm2; 37C, 5% CO2, mycoplasma contamination eliminated with BM-cyclin; negative, HOECHST and PCR
Validated by: HLA-DP beta (confirmed as human by PCR) * isoenzymes (confirmed as human with AST)

Bibliographic references:

Pediatr Res 1990;27:1-6 - PMID: 2296463