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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: ATL98009
Continuous culture, grown as suspension
semi-adherent, spherical morphology
SPECIES: mouse, AKR/J
TISSUE/ORGAN: thymus
TRANSFORMED BY: X-rays Back To Radiant Agents' WebPage
DEPOSITOR: Dr D. Iannelli, Immunology Dept, Faculty of Agriculture, Naples, Italy
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: the cells express Lyt-2, Lyt-3 and CD4 in the cytosol, but not on the membrane; they do not reexpress Lyt-2, Lyt-3 and CD4 on the membrane after sorting by magnetic particles (5 cycles)
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; Freezing medium: Culture medium + 50% FBS + 10% DMSO; maintain cultures between 3-9 x 10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mouse with MD, NP)
Bibliographic references:
- Immunogenetics 1994;40(2):154-158 - PMID: 7517914
- J Immunol Methods 1992;154(2):211-216 - PMID: 1401954
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it