BRL 3A

Version 4.0.200811

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BRL 3A (rat, buffalo, liver)

SPECIES: rat, buffalo
TISSUE/ORGAN: liver

ECACC catalog code: 85111503
Morphology: Epithelial
Description: Buffalo Rat liver
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: MSA factors Applications: Growth factor studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Originally isolated by primary cloning serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1442

Bibliographic references:

Cell 1977;11:441-446 - PMID: 302146
J Cell Physiol 1973;81:153 - PMID: 4735141
Proc Natl Acad Sci U S A 1969;62:852-859 - PMID: 4308097