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SPECIES: rat, buffalo
ECACC catalog code: 85111503
Description: Buffalo Rat liver
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: MSA factors Applications: Growth factor studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Originally isolated by primary cloning serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1442
- Cell 1977;11:441-446 - PMID: 302146
- J Cell Physiol 1973;81:153 - PMID: 4735141
- Proc Natl Acad Sci U S A 1969;62:852-859 - PMID: 4308097
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293