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SPECIES: rat, Walker
ECACC catalog code: 88031148
Susceptibility to viruses: herpes simplex * pseudorabies * vaccinia
Description: Rat Walker carcinoma
Viruses: See Properties
Depositor: Dr E I Levene, Department of Pathology, University of Cambridge, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity and virus studies: HSV, PRV and vaccinia viruses
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Karyotype: 2n = 42
Derived from the parent line LLC-WRC 256, which was established in 1954. The cells are tumorigenic in suckling rats and support the growth of HSV, PRV and Vaccinia viruses.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293