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IZSBS catalog code: BS CL 89
Continuous culture, grown as monolayer
SPECIES: human, Caucasian (male) Karyology: 2n = 46 in 40 cells
TISSUE/ORGAN: lung, embryonic
TRANSFORMED BY: SV40
Endogenous viruses: contains SV40
Susceptibility to viruses: herpes simplex * poliovirus 2 * Sindbis * vesic. stomatitis (Indiana)
DEPOSITOR: obtained from ATCC
PROPERTIES: transformation; virology
Available in the following LABORATORY:
Istituto Zooprofilattico Sperimentale (IZSBS, Brescia)
BME (HBSS) + 10% FBS + 2x AA + 2x Vitamins; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2 Passage: 155, mycoplasma negative, culture
Cell line contains T and transplantation antigens of SV40 virus.
Validated by: isoenzymes (confirmed as human with NP, G6PD, MD, AST, LD)
Availability in cell line catalogues: ATCC: CCL 95.1
- J Cell Comp Physiol 1965;65:69-84 - PMID: 5863367
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293