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TUMOR: sarcoma, epitheloid
ECACC catalog code: 94092303
Morphology: Epithelial, spindle and giant cells
Description: Human skin epithelioid sarcoma
Viruses: See Properties
Depositor: Mrs C Helson, St Agnes Hospital, 305 North St, White Plains, New York, USA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53 Products: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) Applications: Study of tumours. Tumourigenic in nude mice.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Karyotype: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 2138, ICLC: HTL97024, DSMZ: ACC 328
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293