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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
Monoclonal continuous culture, grown as monolayer
fibroblast-like and epithelial-like morphology
SPECIES: human, Caucasian (female) Karyology: aneuploidies, hypodiploidies
TISSUE/ORGAN: lung
Original line: WI-38
TRANSFORMED BY: herpes simplex 1
non tumorigenic
PROPERTIES: virology; cell biology
Available in the following LABORATORY:
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Dip. Biologia Molecolare, Universita' degli Studi (SIUVI, Siena)
MEM modified + 5% FBS + Antibiotics + NaHCO3; Freezing medium: MEM + 20% FBS + 7.5% DMSO; 37C, 5% CO2, split 1-8 Passage: 108, mycoplasma negative, enzyme immunoassay (EIA)
Frequent chromatid fragmentations and acentric fragments. Presence in almos all cells of a large acrocentric chromosome.
Validated by: chromosome analysis
Bibliographic references:
- Giornale di Microbiologia 1977;25:100-103
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it