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TUMOR: sarcoma, osteogenic
ECACC catalog code: 90111314
Description: Rat osteosarcoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Cytosol receptors for the bone resorbing steroid hormone 1,25 -(OH)2 D3. Applications: Tumourigenicity and hormone studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
A cloned derivative of a transplantable rat osteogenic sarcoma. The cells retain many of the characteristics of the tumour including responsiveness to parathyroid hormone (PTH) and prostaglandins.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1661
- J Biol Chem 1980;255:4414-4417 - PMID: 6929275
- Lancet 1980;2:401 - PMID: 6105521
- Nature 1976;260:436-438 - PMID: 1062678
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293