U-87 MG

Version 4.0.200811

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U-87 MG (human, Caucasian, brain, glioblastoma-astrocytoma)

SPECIES: human, Caucasian
TISSUE/ORGAN: brain
TUMOR: glioblastoma-astrocytoma

ECACC catalog code: 89081402
Morphology: Epithelial-like
Description: Human glioblastoma astrocytoma
Viruses: See Properties
Depositor: Dr J Clarke, AVRI, Pirbright, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Neural studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Passages: 4; Karyotype: 2n = 46
Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: HTB 14

Bibliographic references:

Acta Pathol Microbiol Scand 1968;74:465-486 - PMID: 4313504