This is the complete description of the cell line that you requested.
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SPECIES: bovine, bos taurus cow
TISSUE/ORGAN: lymph node
ECACC catalog code: 86062401
Description: Bovine leukaemic lymphoblast
Viruses: See Properties
Depositor: Dr G Theilen, School of Veterinary Medicine, University of California, Davis, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe patent claims.
Properties: Applications: Virus studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Fetal Bovine Serum (FBS). Maintain cultures between 5-9x100,000 cells/ml; 5% CO2; 37C. Cells should not be over diluted.
Karyotype: 2n = 60, aneuploid, modal No 65
BL-3 was established from a neoplastic lymph node of a 3 month old male calf. BVDV positive.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 8037
- J Natl Cancer Inst 1968;40:737 - PMID: 4171552
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293