BL-3

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

BL-3 (bovine, bos taurus cow, lymph node, leukemia)

SPECIES: bovine, bos taurus cow
TISSUE/ORGAN: lymph node
TUMOR: leukemia

ECACC catalog code: 86062401
Morphology: Lymphoblast
Description: Bovine leukaemic lymphoblast
Viruses: See Properties
Depositor: Dr G Theilen, School of Veterinary Medicine, University of California, Davis, USA
No restrictions. Patent: This material is cited in a US and/or other Patent and may not be used to infringe patent claims.
Properties: Applications: Virus studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Fetal Bovine Serum (FBS). Maintain cultures between 5-9x100,000 cells/ml; 5% CO2; 37C. Cells should not be over diluted.
Karyotype: 2n = 60, aneuploid, modal No 65
BL-3 was established from a neoplastic lymph node of a 3 month old male calf. BVDV positive.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 8037

Bibliographic references:

J Natl Cancer Inst 1968;40:737 - PMID: 4171552

For information, get in touch with:

Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it