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TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, acute monocytic
ECACC catalog code: 88081201
Susceptibility to viruses: HIV-1
Description: Human monocytic leukaemia
Viruses: See Properties
Depositor: Dr J Clarke, AVRI, Pirbright, UK
No restrictions. Patent: None Specified By Depositor
Properties: Receptors: Fc, C3b
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS). Maintain cultures between 2-9x100,000 cells/ml; 5% CO2; 37C Requires glycerol as cryoprotectant.
Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-naphthyl butyrate esterase produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Con A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: TIB 202
- Cancer Res 1982;42:1530 - PMID: 6949641
- Int J Cancer 1980;26:171-176 - PMID: 6970727
- J Immunol 1983;131:1882 - PMID: 6619543
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293