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SPECIES: mouse, SIM
ECACC catalog code: 85011409
Susceptibility to viruses: SV40
Description: Mouse SIM embryo fibroblast
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Contact inhibition, transformation and virus studies: SV40
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (ECACC No. 90030801). Demonstrates a high degree of contact inhibition.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- J Cell Physiol 1968;72:141-148 - PMID: 4301006
- Science 1968;162:1024-1026 - PMID: 4301647
- Virology 1965;27:179-185 - PMID: 4284655
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293