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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
SPECIES: dolphin, Stenella plagiodon
TISSUE/ORGAN: kidney
ECACC catalog code: 89120705
Morphology: Epithelial
Susceptibility to viruses: adenovirus 5 * bovine diarrhea virus * calicivirus * Coxsackie A9 * Coxsackie B5 * herpes simplex * hog cholera virus * infectious bovine rhinotracheitis * influenza A * parainfluenza 3 * Powassan encephalitis * reovirus 3 * St Louis encephalitis * vaccinia * vesicular stomatitis (Ogden strain) * western equine encephalitis
Description: Dolphin kidney
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: a wide range of mammalian viruses
Available in the following LABORATORY:
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Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 11;
Karyotype: 2n = 44
From adult male Atlantic spotted dolphin (Stenella plagiodon). Susceptible to wide range of terrestrial mammalian viruses: Ad5, Coxsackie A9+B5, Reovirus3, Vaccinia VSV (Ogden strain), BVD, IBR, Influenza A, parainfluenza3, hog cholera (Ames strain), Western equine encephalitis, St Louis encephalitis and Powassan viruses. Also known as Sp1K (NBL-10).
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CCL 78
Bibliographic references:
- Am J Vet Res 1968;29:1033 - PMID: 4967124
- In Vitro 1966;2:132
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it