This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ECACC catalog code: 94050409
Description: Rat embryo
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 5% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:4 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
RAT-2 was derived from a subclone of a 5'-bromo-deoxyuridine (BUdR)-resistant strain of the Fischer rat 3T3-like cell line RAT-1. RAT-2 lacks appreciable levels of nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confrimation)
Availability in cell line catalogues: ATCC: CRL 1764
- Virology 1981;113:408-411 - PMID: 7269249
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293