This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
TISSUE/ORGAN: brain, fetal
ECACC catalog code: 93040119
Description: Rat, BDIX, fetal brain, pre-malignant
Viruses: See Properties
Depositor: Dr J Roscoe, Dept. of Histopathology, University College and Middlesex School of Medicine, London, UK (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Study of changes in the latent period of carcinogenesis
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 15% Fetal Bovine Serum (FBS). Split confluent cultures 1:5 to 1:10 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 10-15% CO2; 37C.
Produced by passaging the latent period line BE10-Early (93040118) beyond passage 17. The cells possess elevated levels of tissue plasminogen activator activity, are not tumourigenic in BDIX rats and do not form colonies in agar. Do not be use beyond passage 40 as cell properties alter. The BE10 culture was originally derived 2 days after a single dose of ethylnitrosourea to pregnant rats. The control culture BE11 was derived 2 days after exposure to buffer.
Research council deposit: Yes;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Br J Cancer 1980;42:756 - PMID: 7193044
- Carcinogenesis 1986;7:477 - PMID: 3081277
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293