QT 35

Version 4.0.200811

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QT 35 (quail, Japanese, fibrosarcoma)

SPECIES: quail, Japanese
TUMOR: fibrosarcoma

ECACC catalog code: 93120832
Morphology: Fibroblast
Susceptibility to viruses: arbovirus * avian birnavirus * avian reticuloendotheliosis * avian sarcoma virus * coronavirus * paramyxovirus * poxvirus * reovirus * retroviruses * Rous sarcoma virus * turkey herpes
Description: Quail Japanese fibrosarcoma
Viruses: See Properties
Depositor: Dr M Ferrari, Instituto Zooprofilattico, Brescia, ITALY
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C.
Passages: 54; Karyotype: 2n = 78
Derived from methylcholanthrene-induced fibrosarcoma of Japanese quail. The cells are susceptible to several viruses including turkey herpes virus, Rous sarcoma virus and avian sarcoma virus.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: IZSBS: BS TCL 53

Bibliographic references:

Avian Dis 1981;25:839 - PMID: 6279072
Avian Dis 1982;27:261
Avian Dis 1988;32:282 - PMID: 3135794
Cell 1977;11:95 - PMID: 194709