BC3H1

Version 4.0.200811

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BC3H1 (mouse, brain, tumor)

SPECIES: mouse
TISSUE/ORGAN: brain
TUMOR: tumor

DSMZ catalog code: ACC 171
Morphology: adherent, flat cells forming a confluent monolayer
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Description: mouse brain tumor established from a mouse tumor induced by nitrosoethylurea with properties characteristic of muscle; cells were described to synthesize adenylate and creatine phosphokinases confirmed as mouse with IEF of AST, PEP B
Viruses: ELISA. reverse transcriptase positive
Depositor: Dr. J. Wehland, GBF, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
80-90% Dulbecco's MEM + 10-20 % FBS split confluent culture 1:5 every week or 1:10 every two weeks (cells grow slowly) using trypsin/EDTA at 37C with 5% CO2 about 1-2 x 106 cells/80 cm 2; frozen with 70% medium, 20% FBS, 10% DMSO at about 0.5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: isoenzymes (confirmed as mouse with IEF of AST, PEP B)

Availability in cell line catalogues: ATCC: CRL 1443, ECACC: 86093001, IZSBS: TCL 145

Bibliographic references:

J Biol Chem 1977;252:2143-2153 - PMID: 845167
J Cell Biol 1974;61:398-413 - PMID: 4363958