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TUMOR: brain tumor
ECACC catalog code: 86093001
Morphology: Fibroblast - 2 distinct morphologies (see comments).
Description: Mouse brain tumour
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Acetylcholine Applications: Acetylcholine receptor studies.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 20% Fetal Bovine Serum (FBS). Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea and possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1443, DSMZ: ACC 171, IZSBS: TCL 145
- J Biol Chem 1977;252:2143-2153 - PMID: 845167
- J Cell Biol 1974;61:398-413 - PMID: 4363958
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293