BB

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

BB (fish - Brown Bullhead, trunk)

SPECIES: fish - Brown Bullhead
TISSUE/ORGAN: trunk

ECACC catalog code: 87101201
Morphology: Fibroblast
Susceptibility to viruses: channel catfish virus * infectious pancreatic necrosis * vesic. stomatitis (Indiana)
Description: Fish Brown Bullhead posterior trunk tissue
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Virus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virus

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 23C. NB: The cells do not grow well on glass.
Passages: 120; Karyotype: 2n = 60
Derived from trunk tissue (excluding fins) of 2 yr. old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34C. Optimal temperature range for cell proliferation is 25-30C BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.
Research council deposit: No; Price Code: C;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CCL 59, IZSBS: BS CL6

Bibliographic references:

Fish Cell and Tissue Culture;5:253
Fish Physiology 3 1969;Academic Press N Y 1969, 5:253 - PMID: 10140
In Vitro 1980;16:168 - PMID: 6767657