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SPECIES: mouse, SIM
ECACC catalog code: 85061802
Description: Mouse SIM teratocarcinoma
Viruses: See Properties
Depositor: Dr E Robertson, Department of Genetics, University of Cambridge, UK
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Differentiation, growth factors and tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 3-9x100,000 cells/ml ; 0.25% trypsin may be required to detach cells from the feeder layer, which is important for survival during transfers; 5% CO2; 37C.
PSM is derived from the same tumour as PSA-1 (85011403). It differentiates well but requires STO feeder layers for growth.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293