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SPECIES: mouse, NIH 3T3
ECACC catalog code: 94090901
TRANSFORMED BY: Moloney-MuLV
Description: Mouse NIH 3T3 transformed with Moloney murine leukaemia mutants
Viruses: See Properties
Depositor: Dr J Liechty / Dr S K Spratt, Somatix Therapy Corporation, Alameda, California, USA
No restrictions. Patent: None Specified By Depositor
Properties: Products: Amphotrophic helper-free recombinant retroviral packaging cell line. Applications: Amphotropic helper-free recombinant retroviral packaging
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS) (NB. The results obtained by the depositor were gained using 10% Calf Serum (CS)). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5-10% C02, 37C.
From a set of packaging cell lines useful for generating helper-free recombinant retroviruses with amphotropic host ranges. Two mutant Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions were sequentially introduced into NIH 3T3 cells by co-transformation. Each genome contained a deletion of the Psi sequence necessary for the efficient encapsulation of retroviral genomes into virus particles and additional alterations at the 3'end of the provirus. Psi-CRIP can be used to isolate clones that stably produce high titres (1000,000cfu/ml) of recombinant retroviruses with amphotropic host ranges. Viral producers derived from Psi-CRIP do not transfer the packaging function or yield helper virus.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
- Proc Natl Acad Sci U S A 1988;85:6460 - PMID: 3413107
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293