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ECACC catalog code: 95012613
TRANSFORMED BY: SV40
Description: Human prostate normal, immortalized with SV40
Viruses: See Properties
Depositor: Dr P Berthon, Dr O Cussenot, H˘pital SAINT-LOUIS, Paris, FRANCE (Originator)
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Biology of prostate cells, model of differentiated prostate epithelial cells.
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
RPMI 1640 + 2mM Glutamine + 6 -10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C.
Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein. They possess a well differentiated morphology with the expression of cytokeratin 8,18 and 19 with the latter being a feature of differentiated luminal cells of the glandular prostate. Cytokeratin 14, a marker of epithelial basal cells, is not expressed. The cells are non-tumourigenic in nude mice.
Research council deposit: No;
Price Code: C;
Validated by: isoenzymes (species confirmation)
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293