PC-3

Version 4.0.200811

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PC-3 (human, Caucasian, prostate, adenocarcinoma, grade IV)

SPECIES: human, Caucasian
TISSUE/ORGAN: prostate
TUMOR: adenocarcinoma, grade IV

ECACC catalog code: 90112714
Description: Human Caucasian prostate adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 7% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.
Passages: 25; Karyotype: 2n = 46, triploid, modal No 62
Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-alpha reductase activity.
Research council deposit: No; Price Code: A;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: CRL 1435, ICLC: HTL97018, DSMZ: ACC 465

Bibliographic references:

Cancer Res 1980;40:524 - PMID: 7471073
Invest Urol 1979;17:16 - PMID: 447482