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SPECIES: human, Caucasian
TUMOR: adenocarcinoma, grade IV
ECACC catalog code: 90112714
Description: Human Caucasian prostate adenocarcinoma
Viruses: See Properties
Depositor: Obtained from ATCC, USA
No restrictions. Patent: None Specified By Depositor
Properties: Applications: Tumourigenicity studies
Available in the following LABORATORY:
Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
Coon's modified Ham's F12 + 2mM Glutamine + 7% Fetal Bovine Serum (FBS). Split confluent cultures 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.
Karyotype: 2n = 46, triploid, modal No 62
Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-alpha reductase activity.
Research council deposit: No;
Price Code: A;
Validated by: isoenzymes (species confirmation)
Availability in cell line catalogues: ATCC: CRL 1435, ICLC: HTL97018, DSMZ: ACC 465
- Cancer Res 1980;40:524 - PMID: 7471073
- Invest Urol 1979;17:16 - PMID: 447482
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293