P3X63Ag8

Version 4.0.200811

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P3X63Ag8 (mouse, BALB/c, myeloma)

SPECIES: mouse, BALB/c
TUMOR: myeloma

ECACC catalog code: 85011401
Morphology: Lymphoblast
Description: Mouse myeloma
Viruses: See Properties
Depositor: Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford, UK
No restrictions. Patent: None Specified By Depositor
Properties: Products: Immunoglobulin G (IgG1) (kappa) Applications: Fusion partner

Available in the following LABORATORY:

Part of the Business Division of the Health Protection Agency (ECACC, Salisbury, Wiltshire)
DMEM or RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO2; 37C. Cells will achieve maximum cell density of 5x100,000 cells/ml.
Karyotype: Aneuploidy
Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20痢/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.
Research council deposit: No; Price Code: B;
Validated by: isoenzymes (species confirmation)

Availability in cell line catalogues: ATCC: TIB 9, ICLC: ATL05001

Bibliographic references:

Eur J Immunol 1976;6:511-519 - PMID: 825377
Exp Cell Res 1970;60:61-77 - PMID: 5439579
Nature 1975;256:495 - PMID: 1172191
Science 1964;145:709