P-815

Version 4.0.200811

This is the complete description of the cell line that you requested. By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.

P-815 (mouse, DBA/2, mastocytoma)

SPECIES: mouse, DBA/2
TUMOR: mastocytoma

DSMZ catalog code: ACC 1
Morphology: single, round cells in suspension; some cells may be adherent
Transformed by: Chemical Agent Back To Chemical Agents' WebPage
Description: mouse mastocytoma established from the mastocytoma tumor of a DBA/2 mouse treated with methylcolanthrene; used as target cells for cytotoxic T cell assays; as reported cells exhibit no effector activity in an antibody-dependent cell mediated cytotoxic system confirmed as mouse with IEF of LDH, NP, PEP B
Viruses: ELISA. reverse transcriptase negative
Depositor: Dr. H. G. Drexler, DSMZ, Braunschweig, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-5 x 105 cells/ml, cells grow very fast; split 1:5 to 1:10 every 3-4 days at 37C with 5% CO2 maximum cell density at > 1 x 106 cells/ml; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization assays;
Validated by: cytogenetics 1 (near-diploid karyotype; 41(36-43)<2n>; 1-2 centric fusion marker present) * isoenzymes (confirmed as human with IEF of LDH, NP, PEP B)

Availability in cell line catalogues: ATCC: TIB 64

Bibliographic references:

Biochem Biophys Res Commun 1974;61:1268-1275 - PMID: 6616699
Cancer Res 1977;37:546-550 - PMID: 318922
Cell Immunol 1973;9:60-66 - PMID: 4270287
J Exp Med 1976;143:1528-1533 - PMID: 1083890
J Immunol 1973;111:389-394 - PMID: 4123976
J Immunol 1977;119:950-954 - PMID: 894031
J Natl Cancer Inst 1957;18:587
Nature 1974;249:49-51 - PMID: 4208429