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Monoclonal continuous culture, grown as monolayer
SPECIES: mouse, 129/SV (male)
tumorigenic in syngeneic animals
PROPERTIES: pluripotent; differentiation
Available in the following LABORATORY:
Istituto Nazionale per la Ricerca sul Cancro (GEICV, Genova)
DMEM + 10% FBS + 2mM L-Glutamine; Freezing medium: Culture medium + 10% DMSO; 37C, 5% CO2, split 1-10, mycoplasma negative
Embryonal carcinoma cells. Derived from spontaneous testicular tumor. Growth on gelatin-coated dishes. Differentiation into visceral or parietal endoderm with retinoic acid or hexame thylenebisacetamide treatment. LTR blocked, permissive after differentiation. Obtained from Dr. M.I. Sherman Nutley, N.J.
Availability in cell line catalogues: ATCC: CRL 1566, ICLC: ATL06001
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293