B95-8

Version 4.0.200811

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B95-8 (monkey, marmoset, lymphocyte B, peripheral)

SPECIES: monkey, marmoset
TISSUE/ORGAN: lymphocyte B, peripheral

DSMZ catalog code: ACC 100
Morphology: single, round to oval cells or clustered in small clumps in suspension and partially adherent; ca. 10% adhere, but trypsin is not necessary to detach the cells
TRANSFORMED BY: Epstein-Barr
Endogenous viruses: contains Epstein-Barr
Description: marmoset monkey lymphocytes established from peripheral blood lymphocytes of a marmoset monkey (saguinus oedipus); cells were described to release high titers of Epstein-Barr virus (EBV) providing a source for transformation of B cells and establishment of continuous cell lines; this cell line has been assigned by the ZKBS (German Central Commission for Biological Safety) to risk group 2 IEF analysis with LDH and NP confirmed the cells as non-human and non-murine
Viruses: ELISA: reverse transcriptase negative; PCR: EBV+, HBV-, HCV-, HIV-, HTLV-I/II-
Depositor: Prof. Dr. M. Hadam, Medical School of Hannover, Hannover, Germany
General Restrictions

Available in the following LABORATORY:

German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig)
90% RPMI 1640 + 10% FBS maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:5 every 72 hours; minimal density at 0.2 x 106 cells/ml; adherent cells might be detached by tapping the flask; seed out at ca. 0.5 x 106 cells/ml at 37C with 5% CO2 maximal density at about 3 x 106 cells/ml; doubling time of ca. 41 hours; frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 106 cells/ampoule; negative in DAPI, microbiological culture, RNA hybridization, PCR assays;
Validated by: cytogenetics 1 (near-diploid simian karyotype with 4% polyploidy; 44(42-47)<2n>; chromosome rearrangements present) * DNA fingerprinting (unique Hinf I-(gtg)5 DNA profile) * isoenzymes (confirmated as non-human and non-murine with LDH, NP) * reverse transcriptase (reverse transcriptase negative)

Availability in cell line catalogues: ATCC: CRL 1612, ECACC: 85011419

Bibliographic references:

Proc Natl Acad Sci U S A 1973;70:190-194 - PMID: 4346033