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Version 4.0.200811 |
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This is the complete description of the cell line that you requested.
By selecting a generic term (e.g. SPECIES) you will go to the corresponding list of controlled terms, while by selecting a specific term you will go to the list of corresponding cell line names.
ICLC catalog code: AL97002
Monoclonal continuous culture, grown as monolayer
fibroblast-like morphology
SPECIES: mustela vison (mink)
TISSUE/ORGAN: lung, fetal
Susceptibility to viruses: herpes simplex * reovirus 3 * vaccinia * vesicular stomatitis (Ogden strain)
DEPOSITOR: Dr O.E. Varnier, Molecular Virology Unit, ABC, Genoa, Italy.
Cell line available for distribution. For non-commercial investigative use only
PROPERTIES: virology
Available in the following LABORATORY:
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Istituto Nazionale per la Ricerca sul Cancro c/o CBA (ICLC, Genova)
MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1% non-essential amino acids; Freezing medium: Culture medium + 50% FBS + 10% DMSO; Split confluent cultures 1:2-1:4 using trypsin/EDTA; seed at 2-4 x 104 cells/cm2; 37C, 5% CO2. Passage: 40, mycoplasma negative, HOECHST and PCR
Validated by: isoenzymes (confirmed as mink with MD, Pep B)
Bibliographic references:
- In Vitro 1983;19:7 - PMID: 6337087
- Virology 1974;60:282-287 - PMID: 4366800
- Virology 1975;67:344 - PMID: 52940
For information, get in touch with:
Istituto Nazionale per la Ricerca sul Cancro,
c/o Centro Biotecnologie Avanzate, Torre B - Piano 2
Largo Rosanna Benzi, 10 - I-16132 Genova - Italy
Tel: +39-0105558-289, Fax: +39-0105558-293
Email: assunta.manniello@istge.it